半边旗提取物5F联合5-Fu对A/J小鼠诱发性肺癌的影响

摘要

目的：研究半边旗提取物5F 与5-氟尿嘧啶合用对 A/ J 小鼠诱发性肺癌的影响。方法以苯并[a]芘和甲基亚硝胺吡啶基丁酮混合剂灌胃，每周1次，连续8周，诱发小鼠肺癌。从第9周开始，分别腹腔注射5F、5-氟尿嘧啶或（5F + 5- 氟尿嘧啶），连续给药14周后处死小鼠。观察肺癌数量，计算肿瘤抑制率，检测肝脏系数、肾脏系数，检测血清丙氨酸氨基转移酶、天门冬氨酸氨基转移酶、尿素氮和血肌酐水平，检测超氧化物歧化酶和丙二醛的含量变化。结果5F 与5-氟尿嘧啶联合用药显著抑制 A/ J 小鼠诱发性肺癌，具有协同效应，且对肝肾功能无明显毒副作用，还可以提高血清超氧化物歧化酶水平。结论5F 与5-氟尿嘧啶合用可协同抑制 A/ J 小鼠诱发性肺癌。%Objective To investigate the effect of 5F,an extract from Pteris semipinnata L,in combine with 5 - Fu,on the model of induced A/ J mice lung cancer. Methods Intragastric administration was conducted on all mice using mixed re-vulsant of B[a]P and NNK. The aforementioned operation was performed once per week and continued for 8 weeks. 5F and 5- Fu,alone or in combination,were injected into mice from the ninth week and continued for 14 weeks. Count the number of lung cancer and calculate the tumor inhibition rate. Weight the liver and kidney,and calculate the organ index. The levels of ALT,AST,BUN,Scr,SOD and MDA were also detected. Results The combined application of 5F and 5 - Fu significantly suppressed the development of B[a]P and NNK - induced lung cancer in vivo with minimal side effects,and increase the level of serum SOD. Conclusion 5F combined with 5 - Fu had a synergistic effect on the induced lung cancer in A/ J mice.