Objective To establish an HPLC method for the determination of swertiamarin and chiratin in Swertia ja-ponica,as well as to evaluate the quality of S. japonica samples from China and Japan. Methods The determination method was developed on Phenomenex C18 column,eluting by a mixture of acetonitrile and water in gradient. The detector was set at 238 nm for swertiamarin and 247 nm for chiratin. Results The line ranges of swertianmarin and chiratin were 0. 019 ~1. 234 mg·mL-1(r=1)and 0. 002~0. 232 mg·mL-1(r=0. 999 8),respectively. The average recoveries were 99. 50%( RSD=1. 58%)and 99. 90%( RSD=2. 10%),respectively. The HPLC chromatograms of S. japonica samples from Japan and China were similar except for the relative height of some peaks. Compared with samples from Japan,S. japonica cultivat-ed in China contained more swertiamarin and less chiratin. Both swertiamarin and chiratin were present with high content in flowers of S. japonica. Conclusion S. japonica was rich with swertiamarin as a potential medicinal resource. The quantita-tive method can be used in the development and quality control of S. japonica in China.%目的：建立一种可以同时测定日本当药中獐牙菜苦苷与当药苷的方法，对我国引种与日本产的日本当药进行品质评价和比较。方法采用高效液相色谱法，色谱柱为Phenomenex C18（4.6 mm ×250 mm，5μm）；流动相为乙腈-水，梯度洗脱，流速为1 mL·min-1，柱温为30℃，进样量为10μL，检测波长分别为238、247 nm。结果獐牙菜苦苷、当药苷分别在0.019~1.234 mg·mL-1（r=1）、0.002~0.232 mg·mL-1（r=0.9998）内呈良好的线性关系，平均加样回收率分别为99.50%、99.90%，RSD值分别为1.58%、2.10%。我国引种日本当药与日本产样品相比，个别成分相对含量略有差异，其中獐牙菜苦苷含量较高，达到了97.21 mg·g-1，而当药苷含量则较低；不同产地的日本当药均以花中有效成分含量最高。结论我国引种的日本当药中的獐牙菜苦苷含量高，很有开发价值，该文所建方法为我国对国产日本当药的合理开发利用和质量控制提供了依据和技术。
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