骨桥蛋白在原发性干燥综合征患者唇腺中的表达

摘要

Objective : To investigate the mRNA transcription and protein expression of osteopontin ( OPN) and to analyze the possible role of OPN in primary Sjogren' s syndrome ( pSS). Methods: In this study, 24 patients with pSS were selected and diagnosed according to the American-European Consensus Group criteria. The control group was composed of 14 subjects. Total RNA from labial glands was extracted. The target gene and |3-actin acted as templates to perform reverse transcript polymerase chain reaction ( RT-PCR). Then mRNA expression of the target gene were semi-quantitated by the OD ratio of the target gene to p-actin bands on gel under densitometry. Immunohistocheical analysis was used to determine the expression of proteins of the target genes in labial glands from patients and healthy control. Results: In contrast to the control group, the mRNA transcription and protein level of the target genes in labial glands in pSS patients were significantly increased statistically (P < 0. 05 ). Spearman and Pearson rank correlation coefficients were used for analyzing the correlation of lymphocytes foci-score ( LFS) with OPN expressions, the level of the mRNA transcription (0.407, P= 0.049) and protein expression of OPN (0.476, P ?0.039) was positively correlated with the LFS. Immunohistochemistry detected that OPN protein was not only mainly expressed in intracytoplasm of the gland duct, but also in infiltrated lymphocytes. Conclusion; OPN is higher expressed in the basal and surface of labial glands of pSS patients , which may contribute to the destruction of labial glands.%目的:研究骨桥蛋白( osteopontin,OPN)在原发性干燥综合征(primary Sj(o)gren's syndrome,pSS)患者唇腺组织中的表达情况.方法:收集24例pSS患者和14例对照者的唇腺标本,提取唇腺中总RNA,利用反转录聚合酶链反应(RT-PCR)法扩增OPN、β-肌动蛋白(β-actin)的片段,计算OPN/β-actin光密度比值.采用免疫组织化学染色方法检测OPN蛋白在唇腺中的表达情况.结果:pSS组唇腺中OPN的mRNA转录及蛋白表达水平均高于对照组(P＜0.05).OPN表达水平与淋巴细胞灶性评分(lymphocyte foci-score,LFS)呈正相关,mRNA转录水平与LFS相关系数为0.407(Pearson相关系数),P=0.049；蛋白表达程度与唇腺LFS相关系数为0.476(Spearman相关系数),P=0.039.免疫组织化学染色显示,OPN蛋白主要分布在腺上皮细胞胞浆内,但在浸润的淋巴细胞胞浆中也有阳性表达.结论:pSS患者唇腺中OPN表达增加,可能是唇腺结构破坏的原因之一.