Objective: The present study was proposed to investigate in vivo osteogenic potential of bone marrow stromal cells (BMSCs) in negative pressure treated β-Tricalcium phosphate β-TCP) scaffolds. Methods: TCP was dealt with the syringe system (made for a subatmospheric pressure), cocultured or not cocultured with BMSCs, and differentiate into osteogenic direction for 2 weeks. BMSCs sheet was packed into TCP scaffolds and embedded subcutaneously in nude mice for 8 weeks. Animals were sacrificed, osteogenic potential were observed histologically by HE, and Masson stain. Results: TCP cocultured with BMSCs packed with cell sheets showed better osteogenic potential in vivo . Conclusion: Because of the formation of extracellular matrix, the negative pressure and cell sheets may promote cell colonization and improve bone formation in vivo.%目的:研究经过负压处理的TCP支架材料与细胞复合后的体内成骨能力.方法:将TCP支架材料负压处理,和骨髓基质细胞(BMSCs)细胞共培养一段时间后,成骨诱导2周,电镜下观察支架材料和细胞复合情况.再将该复合物与BMSCS细胞膜片复合,植入到裸鼠皮下,8周后作组织切片,HE染色和Masson染色,观察其体内成骨能力.结果:负压(有细胞)+膜片组异位移植8周后,内部可见大量胶原纤维和成骨纤维,在团块样组织内部可见大量成骨细胞,并有血管形成.对照组仅有个别细胞密集区,胶原纤维和成骨纤维较少.空白组未见类似情况.结论:经过负压+骨髓基质细胞膜片处理的TCP支架材料,在体内有成骨潜能.
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