99 Tcm（CO）3-BPHRGD的制备及正常鼠体内生物分布

摘要

制备了99Tcm(CO)3-BPHRGD,并进行体内外生物学评价。在 pH=7、75℃条件下反应30 min,99Tcm(CO)3-BPHRGD的标记率均大于80%,纯化后标记物的放射化学纯度大于98%；体外稳定性实验显示,在37℃时,标记物在生理盐水、胎牛血清及半胱氨酸溶液中具有很好的稳定性；正常小鼠体内生物分布数据显示,99 Tc m (CO)3-BPHRGD在血液中清除较快,主要通过肝肾代谢。%99 Tcm(CO)3-BPHRGD was prepared,and the invitro and invivo biological evalu-ations were completed.The labeling yield of 99 Tcm(CO)3-BPHRGD is more than 80% under optimal conditions(pH=7,reacting at 75 ℃ for 30 min),and the radiochemical purity is more than 98% after purified.Stability invitro experiments show that the radiolabeled com-pound shows good stability in physiological saline,fetal bovine serum,and cysteine solution under 37 ℃.The biodistribution of 99 Tcm (CO)3-BPHRGD in normal mice shows that the metabolic pathways are mainly though liver and kidney with rapid clearance in blood.