【目的】利用生物测定和GC-MS分析方法,研究大蒜根系分泌物的化感作用及化感物质。【方法】采用琼脂培和砂培2种栽培方式收集大蒜根系分泌物,分别采用乙醚、乙酸乙酯、三氯甲烷和正丁醇4种有机溶剂进行萃取分离,对各有机溶剂萃取液进行萝卜种子发芽试验,确定出强化感作用组分,并对其成分进行GC-MS分析。【结果】各有机溶剂萃取液的化感作用强弱顺序依次为：乙酸乙酯萃取液〉三氯甲烷萃取液〉正丁醇和乙醚萃取液,初步确定大蒜根系分泌物中的化感物质为2,6-二异丙基苯酚、2,6-二叔丁基对甲酚和二烯丙基二硫醚。【结论】2种栽培方式收集的大蒜根系分泌物的乙酸乙酯组分中的成分相似,但在含量上差异较大,表现为砂培〉琼脂培。%【Objective】 The allelopathy and major allelochemicals in root exudates of garlic were studied by bioassay and GC-MS methods.【Method】 The root exudates of garlic were collected from agar culture and sand culture in this experiment,and were extracted by ethyl ether,ethyl acetate,chloroform and n-butanol.【Result】 The results showed that allelopathy of ethyl acetate component was the strongest,followed by chloroform component,the allelopathy of ethyl ether component and n-butanol component.The component of ethyl acetate was analyzed by GC-MS,and the main allelochemicals were identified as 2,6-bis（1-methylethyl）-phenol,butylated hydroxytoluene and diallyl disulphide.【Conclusion】 The allelochemicals were similar in ethyl acetate fraction of two cultivation forms,but the quantity was different,and the varieties and quantities of allelochemicals in sand culture were higher than agar culture.
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