苹果蠹蛾颗粒体病毒张掖株(CypoGV-zy)的室内毒力测定

摘要

The virulence determination for a new demestic GV strain, Cydia pomonella granulovirus isolated from Zhangye (CypoGV-zy), Gansu Province, China was described.The values of LD50 for the first,second, third and fourth instar larvae were 4.55 × 103 PIB · mL-1 , 1.20 × l04 PIB · mL-1 , 3.43 × 104 PIB · mL-1 , and 2.11 × 105 PIB · mL-1 , respectively.The first to three instar larvae were very sensitive to CypoGV-zy, the corrected mortalities were 100％ at 7-9 d after infection at a concentration of 0.50 × 105PIB · mL-1.The values of LT50 were 3.59 d, 4.07 d and 4.66 d respectively for the first to third instar, and it was 7.60 d for the fourth instar larvae.The values of LT90 were 5.51 d, 5.91 d, 6.53 d and 16.06 d for the first to fourth instar larvae respectively at the same infection condition.The results indicate that the domestic isolate of CypoGV-zy is a potential biological control agent for C.pomonella in China.%对我国首次分离到的1株苹果蠹蛾颗粒体病毒本土株系,苹果蠹蛾颗粒体病毒张掖分离株(CypoGV-zy)进行了室内毒力测定研究.结果表明:1～4龄幼虫期的LD50分别为4.55×103、1.20×104、3.43×104、2.11×105PIB·mL-1.在O.50×105PIB·mL-1病毒浓度下,1～3龄幼虫在感染病毒7～9 d后校正死亡率达100%,LT50分别为3.59、4.07、4.66 d;4龄幼虫LT50为7.60d,1～4龄幼虫的LT90分别为5.51、5.91、6.53、16.06 d.该病毒分离株对我国西北疫区的苹果蠹蛾具有较强的毒力,具有生防应用前景.