An anatomical observation was conducted on rooting process by in vitro culture of apple rootstock M9.Related factors concerning the culture were examined.1)Satisfactory disinfection effects could be achieved with 75 ％ alcohol for 30 s and 0.1％ mercuric chloride for 8 min during the primary culture,and cultured with the medium of MS+ 6-BA 1.0 mg · L-1 + NAA 0.5 mg · L-1.The survival rate was 18.9％.2) For M9,the appropriate enrichment medium was MS+6-BA 1.0 mg · L-1 +IBA 0.1 mg · L-1 in which the efficiency shoots was 6.4 per stem.3) The proper rooting medium for M9 was 1/ 2MS+IBA 0.3 mg · L-1+NAA 0.1 mg · L-1.Its rooting rate reached 85.5％ when cultured in the dark for 5 days and light culture for 35 days.4)The process of M9 plantlet adventitious root development could be divided into three periods:cell division of formation layer,the formation period of adventitious root primordia,differentiation stage of adventitious root.%通过对苹果砧木M9离体培养,筛选该砧木的初代、继代培养、生根培养基,并解剖分析生根进程.结果表明:1)以M9的离体新梢进行初代培养,75％酒精30 s+0.1％升汞8 min消毒效果较佳,较适培养基为MS+6-BA1.0 mg·L-1 +NAA0.5 mg·L-1.成活率为18.9％.2)M9的适合增殖培养基MS+6-BA1.0 mg·L-1 +IBA0.1mg·L-1,有效新梢数达到6.4个·株-1.3)较适生根培养基为1/2MS+ IBA0.3 mg·L-1+NAA0.1 mg·L-1.暗培养5d后,光培养35d,生根率达到85.5％.4)M9试管苗不定根的发育过程可分为3个时期:形成层细胞分裂期,不定根原基形成期,不定根的分化形成期.
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