In this paper, techniques about primary culture, subculture, root reducing and transplant in different apple stocks were discussed by studying in vitro culture of four apple rootstock breeds, including M9, M26, Malusmicromalus Mark. , Mainshupenensis Rehd. The results indicated that,①For all this four apple rootstocks, the medium of MS+6-BA1. 0 mg·L-1 + NAA0.1 mg·L-1 is the best when their shoots cultivated in vitro.②For M9 and M26, the optimum enrichment medium and the optimum rooting medium were MS + 6-BA 1. 0 mg·L-1 +IBA 0. 1 mg·L-1 and 1/2MS+IBA 0. 3 mg·L-1 +NAA0.1 mg·L-1, respectively. The rooting rates were 70. 6% and 69. 6%, respectively. ③To Malus micromalus Mark and Malus hupenensis Rehd, the optimum subculture medium and the optimum rooting medium were MS + 6-BA1. 0 mg·L-1 + IBA0. 3 mg·L-1 and 1/2MS+IBA0. 3 mg·L-1, respectively. Both of the rooting rates were over 80%.④The nursery substrate treatment with 0. 5 mg·L-1 FeSO4 made the survive rate of the root regeneration plant above 90%.%通过对不同苹果砧木M9、M26、八棱海棠、平邑甜茶的离体培养研究,探讨不同砧木的初代、继代培养、生根以及移栽技术.结果表明,①以4种砧木的离体新梢进行初代培养,最佳培养基均为MS+ 6-BA1.0mg·L1+NAA 0.1mg· L-1.②M9与M26的最佳增殖培养基MS+ 6-BA 1.0 mg· L-1+IBA 0.1mg·L-1,最适生根培养基为1/2MS+IBA 0.3 mg·L-1 +NAA0.1 mg·L-1,生根率分别达到70.6%与69.6%.③八棱海棠和平邑甜茶的最佳继代培养基为MS+6-BA 1.0 mg·L-1+ IBA 0.3 mg·L-1,最适生根培养基为1/2MS+IBA0.3 mg·L-1,两个品种的生根率均达到80%以上.④用0.5 nmg·L-1的FeSO4溶液处理育苗基质可使生根苗移栽成活率提高到90%以上.
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