呋喃虫酰肼对舞毒蛾解毒酶活力的影响

摘要

An experiment was conducted to study the insecticidal activity and mechanism of furan tebufenozide and its effects on the activities of detoxifying enzymes by using cabbage leaf dip method to determine the bioactivity of furan tebufenozide against the larvae of Lymantria dispar. The 24 h-LC50 values of furan tebufenozide were 0. 369 mg · L-1 for the third instar larvae and 0. 842 mg ?L-1 for the fifth instar larvae. Sublethal treatment of furan tebufenozide had a significant effect on enzyme activities. Carboxylesterase (CarE) and glutathione-S-transferase (GST) in the third instar larvae increased first and then decreased with the prolonged time, Acid phosphatase (ACP) decreased continually, Alkaline phosphatase (ALP) decreased first, then increased and then decreased again, and Mixed-function oxidases ( MFOs) decreased first and then increased. The activity of detoxifying enzymes in the fifth instar larvae also showed different changing tendencies: CarE decreased first, then increased and then decreased again; GSTs and ACP decreased continually; ALP and MFOs increased first and then decreased. The development time of the third instar larvae was prolonged after continuous treatment with LC10 and LC30 concentrations of furan tebufenozide. The survival rate of the larvae decreased obviously with increasing furan tebufenozide concentration and the prolonged time. These results indicate that furan tebufenozide as a non-steroidal ecdysone agonist has a higher biological activity against L. Dispar, and it could significantly disturb the major detoxifying enzymes in the larvae, indicating that methoxyfenozide has a high toxic effect on L dispar.%为了研究呋喃虫酰肼(JS118)的杀虫活性及作用机理,以叶片药膜法测定了不同龄期舞毒蛾(Lymantria dispar)幼虫的生物活性及其体内解毒酶活性.结果表明:呋喃虫酰肼对舞毒蛾幼虫24h致死中浓度(LC50)分别为3龄0.369 mg.L-1和5龄0.842 mg.L-1,且对幼虫体内的解毒酶存在显著影响.对3龄幼虫体内CarE和GSTs 表现为先诱导后抑制,对ACP表现为持续抑制作用,对ALP表现为抑制－诱导－抑制作用,对MFOs表现为先抑制后诱导作用；对5龄幼虫体内CarE表现为抑制－诱导－抑制作用,对ACP和GSTs表现为持续抑制作用,对ALP和MFOs表现为先诱导后抑制作用.用LC10和LC30剂量呋喃虫酰肼连续处理后,3龄舞毒蛾幼虫的发育时间均明显延长,随着呋喃虫酰肼质量浓度的增大以及药剂处理时间的延长,幼虫的成活率明显降低.上述结果表明,呋喃虫酰肼作为非甾醇类蜕皮激素竞争物中的新型化合物,对舞毒蛾幼虫有较高的生物活性,能明显影响幼虫的生长发育并对其体内主要解毒酶系有较强的干扰作用,表现出很高的毒杀效果.