柽柳硫氧还蛋白过氧化物酶(ThPrx1)基因转入酵母的抗逆能力分析

摘要

从柽柳(Tamarix hispida)中克隆到了一个硫氧还蛋白过氧化物酶(Peroxiredoxin,Prx)家族基因(ThPrx1).为了研究ThPrxl的抗逆功能,将ThPrx1基因构建到酵母表达载体pYES2中,转化到酿酒酵母(Saccharomyces cerevisiae)中,获得重组酵母,并用转空pYES2质粒的酵母作为对照.分别用用NaCl、KCl、高温、低温、CuSO4、CdCl2、山梨醇、Na2CO3、甲基紫精胁迫条件处理转ThPrx1基因酵母(pYES2-ThPrx1)与对照(pYES2)酵母,来检测ThPrx1基因的抗逆能力.结果表明,ThPrx1基因具有抗NaCl、KCl、高温、低温、CuSO4、CdCl、山梨醇、Na2CO3、甲基紫精胁迫的能力.研究结果提示该基因参与了柽柳的抗逆调控过程.%Full-length eDNA of Thioredoxin Peroxidase Gene ( Prxl ) was cloned from Tamarix hispida. To investigate the stress tolerance of PRXl from T. hispida ( ThPrxl ), the recombinant plasmid designated as pYES2-ThPrxl was constructed by inserting the ThPrxl gene into the yeast expression vector pYES2. The plasmid pYES2-ThPrxI was transformed into the yeast Saccharorayces cereuisiae, and the yeast transformed with empty pYES2 was used as the control. The recombinant yeast harboring ThPrxl and the control yeast harboring empty pYES2 were treated with NaC1, KCI, high temperature, low temperature, CuSO4, CdCl2, sorbitol, Na2 CO3 and methyl viologen, respectively. Results indicated that the ThPrxl was tolerant to NaCI, KCI, high temperature, low temperature, CuSO4, CdCl2, sorbitol, Na2CO3 and methyl viologen. ThPrxl might play a role in stress tolerance of T. hispida.