不同刺槐种源根瘤菌16S rDNA的PCR-RFLP分析

摘要

为了研究不同刺槐种源根瘤茵的遗传多样性,采用4种限制性内切酶对分离自保定和高邑的38株不同刺槐种源根瘤茵进行16S rDNA PCR-RFLP多态性分析,共产生26种16SrDNA遗传图谱类型.UPGMA聚类分析结果显示,所有供试菌株分为4大类,第3类又分为3个亚类.16S rDNA全序列分析表明,代表菌株GY-2与S.morelense LMG20571序列相似性达到99.6％,在系统发育分类地位上属于Sinorhizobium.刺槐根瘤菌遗传多样性丰富,遗传差异受地理环境影响较大,与寄主种源相关性不明显.%A study was performed to analyze the genetic diversity of rhizobia isolated from different provenances of Robinia pseud-oacacia. PCR-RFLP polymorphism analysis of 16S rDNA of 38 rhizobia grown in Baoding and Gaoyi was made with four restriction endonucleases. A total of 26 geneticmap patterns were generated from the tested strains. UPGMA cluster analysis indicates that all strains can be clustered into four groups, and the third group into three subgroups. 16S rDNA sequence analysis indicates that the representative strain GY-2 has a similarity level of 99. 6% with Sinorhizobium morelense LMG20571, and belongs to Sinorhizobium in the phylogenetic classification status. Results show that the rhizobia isolated from R. pseudoacacia reveal a high level of genetic diversity, and genetic differences are influenced by geographical environment, but its correlation with host provenance is not obvious.