凉水自然保护区原始红松林与人工林土壤微生物多样性

摘要

采用BIOLOG微平板法，研究了小兴安岭凉水国家自然保护区内原始红皮云杉（ Picea koraiensis Na-kai）红松林、青楷槭（ Acer tegmentosum Maxim．）红松林与人工兴安落叶松（ Larix gmelini Rupr．）林、白桦（ Betula platyphylla Suk．）林土壤微生物多样性特征。结果表明：各林型土壤微生物AWCD值随土层深度与季节变化表现为，在表层与0～10 cm土层中，四季2种原始红松林均高于人工林；在＞10～20 cm土层中以人工林在春、夏、秋三季最高，冬季仍以原始红松林最高。不同土层的3种微生物多样性指数均以青楷槭红松林最高，且显著高于2种人工林。可见，原始阔叶红松林土壤微生物比原始针叶红松林及人工林土壤微生物具有更丰富的多样性。%By BIOLOG microplate method, an investigation was conducted to study the soil microbial diversity characteristics of Picea.koraiensis Nakai, Acer tegmentosum Maxim, artificial Larix.gmelini Rupr.and Betula platyphylla Suk.in Liang-shui National Nature Reserve, Xiaoxing’an Mountain.The soil microbial AWCD values of the forest-type varied with soil depth and seasons:in the surface and 0-10 cm layer of soil, it was highest in two original Korean pine forest throughout the year;in the 10-20 cm layer of soil, it was highest in plantation in spring, summer and autumn, while it was highest in the primitive Korean pine forest in winter;three different soil microbial diversity indexes of all soil layers were highest in Acer tegmentosum Maxim forest, and significantly higher than those of two plantation.Therefore, the soil microbial diversi-ty of original Broad-leaved Korean Pine forest is richer than that of the original coniferous Korean pine forest and planta-tion.