太白米离体愈伤组织诱导及植株再生

摘要

We studied the effect of types of explants and plant growth regulators on callus induction, the effect of plant growth regulators on adventitious shoot formation and rooting, and developed the protocols for plant regeneration via adventitious shoot organogenesis in Notholin hyacinthinum preliminarily.Different explants could induce callus formation.However, there was significant difference in induction percentage, in which the explant derived from scale leaves produced up to 90.5%. The optimal medium for callus induction was MS medium with 0.5 mg· L-1 NAA, and the medium supplemented with 0.1 mg· L-1 NAA was more suitable for callus proliferation.The highest frequencies of adventitious shoot formation (89.8%) was on the MS medium supplemented with 0.1 mg· L-1 TDZ, but the basal medium MS supplemented with 1.0 mg· L-1 6-BA and 0.1 mg· L-1 NAA was in favor of the shoot proliferation and growth.The highest rooting percentage was 91.4%, and the excellent development root systems were in 1/2 MS medium supplemented with 1.0 mg· L-1 NAA.%探讨了外植体类型及植物生长调节剂对太白米愈伤组织诱导的影响、植物生长调节剂对不定芽分化及生根的影响，初步建立了再生植株培养方案。结果表明：不同类型的外植体均可诱导出愈伤组织，但诱导率有显著差异，以鳞叶为外植体诱导愈伤组织的诱导率最高，为90．5％，最适培养基是MS＋0．5 mg· L-1 NAA；愈伤组织在MS＋0．1 mg· L-1 NAA的培养基上增殖效果较好。诱导分化不定芽阶段以 MS＋0．1 mg· L-1 TDZ培养基的诱导率最高，可达89．8％；最适培养基是MS＋1．0 mg· L-16-BA＋0．1 mg· L-1 NAA。诱导生根最适培养基是1/2MS＋1．0 mg· L-1 NAA，诱导率最高，可达91．4％。