春石斛继代培养主要影响因素

摘要

We used mixed buds by inducing stem section of Dendrobium nobile‘Huoniao’ as the materials to study the effects of basic media , plant growth regulators , different levels of N and different organic additives on the plantlets multiplication . Combined with the status of growth, we chose the best multiplication media.The best basic media for multiplication is 1/2MS. Adding 0.10 mg・ L-1 TDZ and 0.10 mg・ L-1 NAA, we can obtain a higher proliferation rate .Compared to N of 1/2MS basic media , changing the proportion and levels of N has certain inhibitory effects on the plantlets multiplication .Adding mashed potato 100 g・ L-1 has a better effect on promoting multiplication .%以春石斛品种‘火鸟’（ Dendrobium nobile ‘Huoniao ’）茎段诱导出的侧芽为试验材料，研究了基本培养基、植物生长调节剂、不同氮素的比例与水平、有机添加物对春石斛丛生芽增殖的影响。结合生长情况，选出最适宜的增殖培养基。结果表明，丛生芽增殖培养适宜的基本培养基为1/2MS；添加0．10 mg・ L-1的TDZ和0．10 mg・ L-1的NAA可得到较高的增殖系数；以1/2MS基本培养基中的氮素为对照，改变氮素的比例与水平，均对春石斛的增殖有一定的抑制作用；有机添加物以添加100 g・ L-1的马铃薯泥促进效果较好。