Acclimation of trichloroethylene (TCE)-degrading anaerobic granular sludge was successful by using upflow anaerobic sludge blanket (UASB) reactor. The influences of temperature, pH and initial concentration of TCE on the degradation characteristics of TCE-degrading anaerobic granular sludge were investigated in serum bottles. The results showed that 35℃was the optimum temperature of the granular sludge, the degradation rate constant was 0.1879, the half-life was 3.69 d, and the TCE degradation rate was 90.15%; 7.2 was the optimum pH of granular sludge, the degradation rate constant was 0.1672, the half-life was 4.15 d, and the TCE degradation rate was 88.74%; Under the conditions of temperature of 35℃, pH of 7.2, and the TCE test concentration range from 14.6 to 73.0 mg·L-1, the smal er the initial TCE concentration was, the faster the degradation rate was; When TCE concentration was 73 mg·L-1, TCE-degrading anaerobic granular sludge stil had higher ability to degrade TCE. After 14 d, TCE could be effectively removed and the final TCE degradation rate was over 80%.%通过在上流式厌氧污泥床（UASB）反应器成功驯化TCE厌氧降解颗粒污泥，于小瓶中进行温度、pH和TCE浓度等对TCE厌氧降解颗粒污泥降解特性影响的试验研究。结果表明，35℃是颗粒污泥最适温度，降解速率常数为0.1879，半衰期为3.69 d，TCE降解率为90.15%；颗粒污泥最适pH为7.2，降解速率常数为0.1672，半衰期为4.15 d，TCE降解率为88.74%；在温度为35℃，pH 7.2条件下，试验浓度范围内（14.6~73.0 mg·L-1），TCE初始浓度越小，降解速率越快，降解率越大；当TCE浓度达到73 mg·L-1时，TCE厌氧降解颗粒污泥仍能以较高速率降解TCE，14 d后TCE均可被有效去除，最终降解率在80%以上。
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