Campylobacter jejuni is a kind of pathogenic bacteria which cause serious damage to the human and animal health. To understand the epidemiological and drug resistance of Campylobacter jejuni in broiler of Harbin, the present study investigated the infection status of Campylobacter jejuni in broiler in Harbin and its surrounding area, meanwhile, detected the drug resistance and expression of resistant genes. The results showed that the average infections of chicken farms were 35.67%. Campylobacter jejuni had different degrees of antibiotic resistance to 13 kinds of antibiotics, which the antibiotic resistance of tetracycline and ampicil in sodium were 100%, while there were multiple drug resistance. The highest drug-resistant spectrums were 11, while the detection rate of bacterial strain was 14.95%. 57.94%bacterial strain contained tetA, tetB, tetC, blaCTX-M, blaTEM and blaNDM-1 genes, that the detection rate were 57.94%, 66.36%, 28.97%, 42.99%, 55.14% and 8.41%, respectively. However, the present study didn't detect tetD and blaSHV-1 genes. The results showed that the infections of Campylobacter jejuni in chicken farms of Harbin were occured commonly, while there were multiple drug resistance. It provided the basis for effective prevention and treatment of Campylobacter jejuni.%空肠弯曲菌是严重危害人畜健康的病原菌,为了解哈尔滨市肉鸡源性空肠弯曲菌流行病学和耐药性,研究调查哈尔滨市周边肉鸡场空肠弯曲菌感染状况,通过耐药性和耐药基因检测,发现鸡场平均感染率为35.67%,对13种抗生素均有不同程度耐药性,四环素和氨苄西林钠达100%,存在多重耐药性,最高耐药谱达11个,检出菌株率为14.95%.57.94%菌株携带tetA、tetB、tetC、blaCTX-M、blaTEM和blaNDM-1基因,检出率分别为57.94%、66.36%、28.97%、42.99%、55.14%和8.41%,而tetD和blaSHV-1基因未检出.结果表明,哈尔滨市肉鸡场空肠弯曲菌感染普遍,存在多重耐药并携带多种耐药基因,为有效防治空肠弯曲菌病提供依据.
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