复方中药对山女鳟受精卵水霉病防治效果的研究

摘要

Effects of five self-made Chinese medical herbs on preventing and control Saproleginiasis of Oncorhynchus masou masou fertilized eggs were evaluated in this study. The active ingredients of Chinese medical herbs here were extracted by two different methods including water decoction and combination of water decoction and ethanol extraction. The normal dosage groups and dilution groups were set up to compare the effects of five different Chinese herbal compounds with two disinfection concentrations. Under the flowing water fish culture mode at 10 ℃, concentrated drugs were added into medicine flasks and dropped into the horizontal tanks to immerse the fertilized eggs for 40 min. The results indicated that among of five kinds of Chinese herbal compounds, Compound A could improve significantly eyed rate of fertilized eggs and decrease their mortalities caused by Saprolegnia infection under the normal dosage, which could be used as a good Chinese herbs preparation to prevent and control Saprolegniasis during the hatching period.%文章对5种自组复方中药的2种提取方法2种消毒浓度，对山女鳟受精卵孵化期间水霉病防治效果进行筛选研究。采用水煎法及水煎+醇提混合法对复方中药有效成分进行提取，并设置正常剂量和稀释消毒组对5种复方中药的消毒效果进行比较研究。在10℃流水养殖条件下，将浓缩的药液加入吊瓶中，逐滴加入流水混匀，平列槽中试验鱼卵药液浸泡约40 min。结果表明，5种复方中药中，复方A水煎剂正常消毒剂量组能显著提高受精卵的发眼率，降低水霉菌感染造成的死亡，是适用于山女鳟受精卵孵化期间防治水霉感染的中药制剂。