深黄被孢霉突变菌株发酵制备花生四烯酸工艺研究

摘要

在单因素试验基础上,采用响应面分析法优化深黄被孢霉突变菌株发酵制备花生四烯酸工艺,确定最优发酵工艺条件为:接种量15.66%,发酵温度28.29℃,发酵时间6.58 d,发酵pH 5.96.花生四烯酸产量为3.12 g·L-1,在最优发酵工艺条件下,可提高产量,同时减少接种量,缩短发酵时间,降低生产成本.%On the basis of single factor experiment, technological parameters of preparation for arachidonic acid by Mortierella isabellina mutant strain were determined by response surface methodology to optimize fermentation conditions as following: inoculum size was 15.66%, fermentation temperature was 28.29 ℃, fermentation time was 6.58 d, fermentation pH was 5.96. In the best fermentation process conditions, ARA yield was 3.12 g·L-1, which enhanced yield. At the same time, inoculum size was decreased, fermentation time was shortened, and production cost was reduced.