蚯蚓受重金属胁迫时,体内立即产生氧化应激反应,确定胁迫过程氧化应激主要指标对污染监测尤为重要.将胁迫试验分短期和长期试验两组,前者试验周期为10d,每天取1条蚯蚓;后者试验周期为30d,每10d取1条蚯蚓.每组均设定Cd2+浓度梯度50、100、125、250和500 mg· kg-1重金属胁迫溶液,选择蚯蚓环带后尾部测定体内SOD、POD、GPX、GST、CAT、VE、MDA和AChE.结果表明,短期试验组,反映蚯蚓体内氧化应激主要指标为CAT、SOD和POD.在长期试验中,反映蚯蚓体内氧化应激主要指标为GPX和MDA.%Earthworms are widely used in all kinds of pollutants as sensitive bio-indicator organisms because of their immediately oxidative stress response under the stress of heavy metal.However,there are a large number of indices associated with the oxidative stress response.Finding out the key monitoring indices in the stress process becomes a practical demand of the pollution monitoring and warning process.The paper studied two groups,the short-term test and the long-term test.The former one lasted 10 d,taking out an earthworm everyday.The latter test lasted 30 d,taking out an earthworm every 10 d.The Cd2+ concentration was set at 50,100,125,250 and 500 mg · kg-1.Post-clitellum segments of earthworms were chosen to determine SOD,POD,GPX,GST,CAT,VE,MDA and AChE.The results showed that the main bio-indicators associating with oxidative stress reaction in short-term group were:CAT,SOD and POD.While with the long-term test,the main bio-indicators associated with oxidative stress reaction were GPX and MDA.
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