HPLC 法测定八味獐牙菜丸中獐牙菜苦苷及龙胆苦苷的含量

摘要

目的：建立八味獐牙菜丸中獐牙菜苦苷及龙胆苦苷的含量测定方法。方法：色谱柱为岛津 VP-ODS C18（250mm×4.6mm，5μm），流动相为甲醇-0.05%磷酸溶液（梯度洗脱），检测波长为237nm。流速为0.8mL·min-1；柱温为30℃。结果：獐牙菜苦苷在0.12～0.60mg·mL-1范围内峰面积与浓度呈良好的线性关系，相关系数为0.9999，平均回收率96.27%，RSD 值为1.52%（n=9）。龙胆苦苷在0.04～0.20mg·mL-1范围内峰面积与浓度呈良好的线性关系，相关系数为0.9999，平均回收率96.24%，RSD 值为1.53%（n=9）。结论：方法快速，准确，重复性好，可作为八味獐牙菜丸的质量指标。%Objective:To establish a HPLC method for the simultaneous determination of Swertimarin and gentiamarin in Bawei Zhangyacai pill. Method: Column for Shimadzu VP-ODS C18(250mm ×4.6mm,5μm),the mobile phase was methanol-0.05% phosphoric acid (gradient elution),the detection wavelength was 237nm. Uses swertiamarin as reference,the flow rate was 0.8mL·min-1;column temperature was 30℃. Results: Swertimarin injection volume in from 0.12~0.60mg·mL-1 range peak area and concentration showed a good linear relationship,the correlation coefficient of 0.9999,the average recovery rate was 96.27% ,RSD is 1.52%(n =9). Gentiamarin injection volume in from 0.02~0.20mg·mL -1 range peak area and concentration showed a good linear relationship,the correlation coefficient of 0.9999,the average recovery rate was 96.24% ,RSD is 1.53%(n =9).Conclusion: The method is fast, accurate,reproducible,and can be used as the indicator of the quality of the Bawei Zhangyacai pill.