HPLC法测定骨康口服液中阿魏酸的含量

摘要

Objective:To establish a quantitative method for ferulic acid in Gukangkoufuye by HPLC.Method: Using a HPLC method with Diamonsil C18 (4.6mm ×250mm,5μm), the mobile phase was acetonitrile-0.085% phosphoric acid solution (17∶83)at a flow rate of 1.0ml·min-1, the UV detection wavelength was 316 nm.Result: The linear range of ferulic acid was in the range between 6.205μg·ml-1 and 24.82μg·ml-1 (r=1.0000). The average recovery of ferulic acid was 98.22% with RSD of 1.4%.Conclusion: This method is simple, efficient and accurate, which can be used for the quality control of ferulic acid in Gukangkoufuye.%目的：建立HPLC测定骨康口服液中阿魏酸的含量测定方法。方法：采用HPLC法测定阿魏酸的含量，Diamonsil C18（4.6mm×250mm，5μm）为色谱柱，流动相为乙腈-0.085%磷酸溶液（17∶83），流速1.0ml·min-1，检测波长316nm。结果：阿魏酸在6.205～24.82μg·ml-1呈良好的线性关系（r=1.0000）。回收率为98.22%（RSD 1.4%，n=9）。结论：本法简便，准确，灵敏度高，重现性好，能有效控制骨康口服液的质量。