HPLC法测定杞菊地黄丸(浓缩丸)中马钱苷的含量

摘要

Objective:To estabilish a method for the determination of of loganin in Qi Chrysanthemum rehmanrha pill (concentrated pill) Method:HPLC C18(4.6mm×250mm,5μm) ,column was used with methanol—acetonitrile—phosphate(0.1%)(5:9:86). The flow rate was 1.0mL/min. The detection wave length was 236nm. The column temperature was at 30℃, and the inject volume was 10μL. Results:The calibration curves showed good linearity in the range of 2μg~0.8416μg (loganin)(r=0.9998).The average recoveries were 99.0%,RSD were1.2%(n=6).Conclusion:This Method is simple, accurate, and could be adopted for the quality control of this durg .%目的:建立测定杞菊地黄丸(浓缩丸)中马钱苷的含量测定方法.方法:HPLC法;C18柱(4.6mm×250mm,5μm),流动相为甲醇-乙腈-0.1%磷酸溶液(5:9:86),流速为1.0mL/min;检测波长236nm;柱温30℃;进样量10μL. 结果:马钱苷的线性范围为2μg~0.8416μg,相关系数为0.9998;平均加样回收率为99.0%,RSD为1.2%(n=6). 结论:该法简单,准确度高,重复性好,可用于控制杞菊地黄丸(浓缩丸)的质量.