氧化苦参碱对青霉素致痫大鼠学习记忆及海马p-JNK表达的影响

摘要

Objective To observe the effect of oxymatrine on the expression of p - JNK in hippocampus and the spatial learning and memory of penicillin - induced epileptic rats, as well as to investigate its mechanisms.Methods 24 SD rats were randomly divided into four groups, which were normal saline control group (NS) ,epilepsy group ( EP), Oxymatraine group ( OXY ) and JNK inhibitor group ( SP600125, SP ).Penicillin was intraperitoneally injected in rats to establish epileptic animal model.Behavioral changes were observed and immunohistochemical method was used to measure the number of positive - cell of p - JNK in hippocampus.The spatial learning and memory was tested by Morris Water Maze (MWM).Results Compared with in EP group, the escape latency, swimming distance, times of crossing platform in OXY group were significantly descreased ( P ＜ 0.05 ); the expression of p - JNK was less in OXY group than that in EP group ( P ＜ 0.05 ).Conclusion The protection of OXY for the spatial learning and memory in epileptic rats is performed by affecting the activation of JNK signaling pathway.%目的 观察氧化苦参碱(Oxymatrine,OXY)对青霉素(Penicillin,PEN)致痫大鼠学习记忆和海马神经元p-JNK表达的影响.方法 成年SD大鼠24只随机分为生理盐水对照组(Normal saline,NS)、癫痫模型组(Epilepsy,EP)、OXY组和JNK抑制剂组(SP600125,SP),腹腔注射青霉素诱导大鼠癫痫发作,观察大鼠行为学改变,Morris水迷宫检测大鼠空间学习和记忆,免疫组化测定海马组织内p-JNK阳性细胞数.结果 与EP组对比,OXY组大鼠逃避潜伏期和穿越平台次数明显减少(P＜0.05);海马组织内p-JNK阳性细胞数明显降低(P＜0.05).结论 OXY可能通过抑制JNK信号通路的活化发挥对癫痫大鼠在空间学习和记忆方面的保护作用.