VEGF-C、VEGFR-3和LYVE-1在胃癌中的表达及其临床意义

摘要

目的 探讨胃癌组织中血管内皮生长因子C(VEGF-C)、血管内皮细胞生长因子受体-3 (VEGFR-3)及淋巴管透明质酸受体-1(LYVE-1)的表达与胃癌临床病理特征的关系及临床意义.方法 应用组织微阵列技术(TMA),免疫组化SP法检测125例胃癌组织标本、96例癌旁正常组织、20例胃良性病变和胃癌癌周600枚淋巴结中VEGF-C、VEGFR-3和LYVE-1蛋白的表达.结果 三指标在不同胃组织中的表达差异均有统计学意义(均P＜0.01);胃癌组织中VEGF-C、VEGFR-3及LYVE-1的阳性表达率分别为62.4%、56.0%及58.4%,明显高于胃良性病变(20.00%、30.0%及25.0%)和癌旁正常组织(10.41%、12.5% 及9.375%)中的表达(P＜0.0083,χ2分割法),与胃癌癌周淋巴结中的表达差异无统计学意义(60%、56%及55%,均P＞0.0083,χ2分割法).VEGF-C、VEGFR-3及LYVE-1三者阳性表达在有无淋巴结转移、远处转移及临床分期间差异有统计学意义 (P＜0.05);Logistic多因素分析显示,VEGF-C 、VEGFR-3和LYVE-1是与胃癌分期和远处转移有关的危险因素.结论 胃癌组织中存在VEGF-C、VEGFR-3和LYVE-1的高表达,与胃癌的发生、发展及侵袭转移有关.%Objective To investigate the expression and clinical significance of VEGF - C, VEGFR - 3 and LYVE - 1 in gastric carcinoma. Methods The immunohistochemical was used to detect the VEGF - C, VEGFR -3 and LYVE -1 in 125 cases with gastric carcinoma, 96 cases with adjacent normal tissue, 20 cases with benign gastric lession and 600 cases with lymph nodes. Results The positive rates of VEGF - C, VEGFR -3 and LYVE - 1 in gastric carcinoma tissue were 62. 4% ,56. 0% and 58.4% and they were higher than those in benign gastric lesion tissue( 20. 0% ,30% ,25% ) and in adjacent normal tissue( 10. 41% ,12. 50% , 9. 375% ) ( P < 0. 0083 ). There was significant difference among the expression of VEGF - C, VEGFR - 3 and LYVE - 1 in lymph node metastasis, distant metastasis and gastric cancer stage( P <0. 05 ). Logistic regression analysis showed that the expression of VEGF - C, VEGFR - 3 and LYVE - 1 increased the risk of gastric cancer staging and distant metastasis. Conclusion The over expression of VEGF - C, VEGFR - 3 and LYVE -1 in gastric carcinoma involved in gastric carcinogenesis and progress of the invasion process.