心脏病患者血清中抗心律失常肽浓度的变化

摘要

为建立血清中抗心律失常肽(antiarrhythmicpeptide,AAP)的放射免疫检测方法；观察心脏病患者血清中AAP分泌变化，部分阐明AAP分泌的影响机制，采用3-(4-羟基苯)丙酸-N-琥珀酰亚胺酯为连接剂，将125Ⅰ标记在AAP上，运用放射免疫方法测定心肌梗死、慢性心力衰竭、心房纤颤患者及对照组血清中AAP的浓度并加以比较。结果显示：心力衰竭、心房纤颤患者血清中AAP的浓度较对照组高，差异有显著性(P＜0.05)；心肌梗死患者血清中AAP的浓度与对照组相比差异无显著性(P＞0.05)。结果表明：AAP的分泌与心肌缺血无关；血流动力学异常会导致AAP分泌增加。%To establish the standard procedure for antiarrhythmicpeptide(AAP)radioimmunoassay,to investigate the effect of cardiac disease on the secration of AAP.and to expound the mechanism of AAP secration partly, N-succinimidyl 3-(p-hroxypheny1)-propinate was used to connect 125Ⅰ and AAP,then thc immune-activity of AAP in serum was determined. The patients was divided into 3 groups:24 cases were with myocardial infarction,26 cases were with chronic heart failure, 23 cases were with atrial fibrillation.20 healthy persons were selected as control group. The dose of AAP in serum of patients were detemined and compared with that of control group.The results showed that the dose of AAP in the serum of patients with chronic heart failure(7.3+0.9 pmol/ml)or atrial fibrillation (4.72±0.98 pmol/ml)were higher than that of control group(3.9±0.6 pmol/ml)(P＜0.05). The dose of AAP in the serum of patients with myocardial infarction(4.01±0.77 pmol/ml) was the same as that of control group.The results suggested:secration of AAP demonstrated no relation to cardiac ischemia,the abnormality of blood flow dynamics could lead to the increase of AAP secration