Objective To explore the effects of fluoxetine on dual stress induced depressive-like and anxiety-like behaviors in Disrupted in Schizophrenia 1 (DISC1) transgenic mice. Methods Sixteen C57BL/6 wild-type mice were randomly assigned into two groups with 8 in each group, the WT-stress group and the WT-control group. Sixteen C57BL/6 LBD-DISC1 transgenic mice were also randomly assigned into two groups with 8 in each, the DISC1-stress group and the DISC1-control group. The WT-stress group and the DISC1-stress group received maternal separation and chronic unpredictable mild stress. All mice were treated with fluoxetine for four weeks. The sucrose consumption, the forced swim test and the open field test were used to evaluate the depressive-like and anxiety-like behaviors. Results There was no significant difference in the immobility time in the forced swim test and the migration distance in the centre area in the open field test among each group of mice after the fluoxetine treatment. The preference for sucrose solution in DISC1-stress group and body weight were still low (P< 0.01). Conclusions Fluoxetine can improve the stress induced anxiety and depression in DISC1 mice. However, it cannot alleviate anhedonia and the loss of body weight.%目的 探讨氟西汀对双重应激诱导的精神分裂症断裂基因1(DISC1)转基因小鼠抑郁和焦虑样行为的影响.方法 C57BL/6野生型新生小鼠及C57BL/6 LBD-DISC1转基因新生小鼠各16只,分别随机分为野生应激组、野生对照组和DISC1应激组、DISC1对照组,每组8只.应激组小鼠进行母婴分离和慢性不可预知轻度应激后,4组小鼠使用氟西汀治疗4周,采用糖水消耗实验、强迫游泳实验及旷场实验测定小鼠行为.结果 氟西汀治疗后各组小鼠旷场实验中心区域移动距离和强迫游泳静止时间之间的差异无统计学意义,DISC1应激组的糖水偏好度和体重仍偏低(P<0.01).结论 氟西汀可改善双重应激诱导的DISC1转基因小鼠的抑郁和焦虑情绪,但不能缓解其快感缺乏和体重降低.
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