琼脂平板及紫外分光光度法筛选林可霉素降解菌

摘要

由于抗生素的残留，林可霉素生产废渣难以处理及资源化利用，这已成为制药企业的一大难题。因此亟需寻找一种简便而又快捷的方法分离林可霉素降解菌。本研究采用林可霉素作为唯一碳源和能源的固体琼脂平板来筛选林可霉素降解菌，通过加热酸溶解琼脂后采用紫外分光光度法测定琼脂平板中的林可霉素含量来考察降解率。结果表明酸溶解加热的最优工艺组合为每10mL培养基的平板中加入3mL浓度为1．14mol／L的盐酸，于80℃加热70s，在该工艺条件下，在0～250μg／mL的浓度范围内，琼脂平板中林可霉素浓度与吸光值呈良好的线性关系，%Due to the existence of antibiotic residues, waste mycelium of lincomycin was difficult to be treated and used as resources, which had become a major problem in the production enterprise. So it is very necessary to develop a simple and rapid method to iso