Screening of lincomycin-degrading bacteria by silica gel plate and visible spectrophotometry

摘要

Due to the existence of antibiotic residues, waste mycelium of lincomycin was difficult to be treated and used as resources, which had become a major problem in the production enterprise. So it is very necessary to isolate and screen lincomycin -degrading bacteria to biodegrade the waste mycelium. In this paper, Box-Behnken Experimental Scheme was performed to optimize the experimental conditions for the treatment of silica gel plates, and the visible spectrophotometry was used to determine the concentration of lincomycin in the silica gel plates to indicate the degradation capacity. The results showed that the optimal conditions were to add 4 ml of water to a silica gel plate, and immerse for 40min, and repeat this process for four times. Under these conditions, the linear correlation between the lincomycin concentration and absorbance was satisfactory in the calibration standards at the range of 0-5mg/ml (r=0.99976). The method precision values (RSD=0.1126％), accuracy values (RSD=0.2358％), reproducibility values (RSD=0.2358％), stability values (RSD=0.1129％) and recovery values (98.1318％) of lincomycin in silica gel aqueous solution were adequate. Application of this method to 1311 strain showed the lincomycin -degradation rate was of 35.81 ±2％. Taken together, we have established a simple, convenient, rapid and valid visible spectrophotometry method to detect lincomycin in silica gel plates for screening lincomycin -degrading bacteria.