MiR-738通过Wnt信号通路调控斑马鱼心脏发育

摘要

目的：研究miR-738对模式动物斑马鱼心脏发育的影响。方法：通过全胚胎原位杂交检测miR-738的表达。在斑马鱼受精卵中显微注射miR-738前体来过表达miR-738。通过显微镜成像来检测心脏表型。通过荧光素酶报告基因检测来验证miR-738调控Lef1。结果：MiR-738在30 hpf斑马鱼心脏表达。 MiR-738过表达导致斑马鱼胚胎心脏显著增大。 MiR-738可以抑制Lef1的表达。结论：MiR-738通过Wnt信号通路调控斑马鱼心脏发育。%Objective:To examine the function of miR-738 involved in zebrafish heat development. Methods:By microinjec-tion of miR-738 precursor into zebrafish fertilized egg to overexpress miR-738. By microscope imaging to check the heart phe-notype. By using luciferase reporter assay to validate that miR-738 regulates Lef1 directly. Results:MiR-738 was detected in zebrafish heart at 30 hpf. Overexpression of miR-738 resulted in the enlargement of zebrafish embryonic heart. MiR-738 in-hibits the Lef1 expression. Conclusion:MiR-738 regulates the zebrafish heart formation through Wnt signaling.