目的：探讨创伤性脑损伤(traumatic brain injury，TBI)后，大鼠脑组织中β-1,4-半乳糖基转移酶Ⅴ(β-1,4-galactosyltransferaseⅤ，β-1,4-GalTⅤ)的表达及作用机制。方法：SD大鼠30只，随机分成假手术组、TBI 1 d、3 d、5 d、7 d组(每组6只)。采用实时定量聚合酶链式反应(real time quantitative polymerase chain reaction, qRT-PCR)检测β-1,4-GalTⅤmRNA的表达变化，Western Blot检测β-1,4-GalTⅤ的蛋白表达变化，免疫荧光双标观察β-1,4-GalT V与神经元标志物NeuN、星形胶质细胞标志物GFAP的共定位情况。结果：TBI后β-1,4-GalTⅤmRNA和蛋白表达水平一致，与假手术组相比，TBI后表达水平均明显升高，并于损伤后3 d达到高峰。免疫荧光双标检测显示，β-1,4-GalTⅤ与NeuN存在明显的共定位，与GFAP存在部分共定位。结论：β-1,4-GalTⅤ在TBI后神经元和星形胶质细胞中均发挥一定的作用，以神经元为主。%Objective: To explore the expression and role mechanism of β-1,4-galactosyltransferaseⅤ(β-1,4-GalTⅤ) in rat brain after traumatic brain injury(TBI). Methods: SD rats(n=30) were divided randomly into sham, 1 d, 3 d, 5 d, 7 d post-TBI group(n=6 per group). Real time quantitative polymerase chain reaction(qRT-PCR) was performed to detect the expression change of β-1,4-GalTⅤmRNA. Western Blot was used to investigate the expression change of β-1,4-GalTⅤprotein. Double-labeling immunofluorescence was used to detect the co-localization of β-1,4-GalTⅤ with NeuN-marker of neuron or GFAP- marker of astrocyte. Results: The results of qRT-PCR are similar to that of Western Blot,which showed that β-1,4-GalTⅤ mRNA increased markedly after TBI compared with sham group and peaked at 3 d post-TBI. Double-labeling immunofluorescence revealed that there was obvious co-localization of NeuN and β-1,4-GalTⅤ. GFAP co-located with β-1,4-GalTⅤpartly. Conclusion: β-1,4-GalTⅤplays a vital role in neuron and astroctye after TBI, mainly in neuron.
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