丹参酮IIA对腹膜透析液诱导的大鼠腹膜组织学变化及CTGF表达的影响

摘要

OBJECTIVE To investigate the effects of tanshinone IIA on the alterations in morphology and the expression of connective tissue growth factor (CTGF) of peritoneal membranes in model rats of peritoneal dialysis. METHODS 50 male Sprague-Dawley (SD) rats were randomly divided into five groups (n=10 in each group). Intraperitoneal injection of 0.9％ saline, standard lactate-buffered 4.25％ glucose-based PDS, and standard lactate-buffered 4.25％ glucosebased PDS with various concentrations of tanshinone IIA (50,100, 200 mg/L) were performed for rats in each group once daily with an instillation volume of 20 mL per injection for 30 days. Afterwards, histological analyses, including hematoxylin and eosin (HE) staining and Masson staining, were carried out in parietal peritoneum. Immunohistochemistry was used to analyze the expression of CTGF. RESULTS As compared with control group, chronic high-glucose-based PDS exposure resulted in increased submesothelial compact zone thickness and accumulation of submesothelial matrix, as well as enhanced expression of CTGF in parietal peritoneum(P＜0.01). The peritoneal changes induced by PDS were significantly ameliorated by tanshinone IIA(P＜0.01). CONCLUSION Long-term exposure to high glucose-based PDS results in damages in peritoneal membranes and promotes the fibrosing process. Tanshinone IIA may be helpful to slow the PDSinduced deterioration of peritoneal membrane.%目的 了解丹参酮IIA对腹腔注射4.25%腹膜透析液(PDS)大鼠腹膜组织学变化以及结缔组织生长因子(CTGF)表达的影响.方法 50只SD雄性大鼠随机分为5组:对照组,每日腹腔注射生理盐水20mL;PDS组,每日腹腔注射4.25% PDS 20mL;丹参酮低、中、高浓度组,每日分别腹腔注射含丹参酮IIA浓度为50mg/L、100mg/L、200mg/L的4.25%PDS20mL.于实验第30天,取壁层腹膜行光镜检查,并用免疫组织化学的方法 检测壁层腹膜CTGF的表达情况.结果 HE、Masson染色显示,在PDS干预下,腹膜显著增厚,胶原沉积显著增多(P<0.01).与PDS组比较,丹参酮组腹膜明显变薄,胶原沉积明显减少(P<0.01).在PDS干预下腹膜CTGF表达较对照组显著增加(P<0.01),丹参酮组CTGF表达与PDS组比较有显著下降(P<0.01).结论 丹参酮II A具有抑制葡萄糖PDS导致的实验大鼠腹膜纤维化及CTGF表达增加的作用.