复肾汤对 db／db 小鼠肾脏病变的影响

摘要

OBJECTIVE To observe the effects of Fushen Decotion on renal lesion in db/db mice.METHODS 8 male C57 mice were chosen as normal control group,conventional breeding.24 male db/db mice with high blood glucose level were di-vided into model group,FST(L1) group and FST(L2) group.Model group mice were given normal saline;L1 and L2 mice were treated with FST 13 g/kg and 39 g/kg,respectively.The weight and blood sugar of mice were measured regularly for 8 weeks.After the experiment,the serum was collected and biochemical parameters,such as T-chol,BUN,SOD and MDA, were detected.Then the kidneys were taken,the expression of VEGF and FN in renal tissues were detected by immunohisto-chemical method,and the renal lesions were observed under light microscope.RESULTS Compared with the model group,T-chol,BUN and MDA of L1 and L2 groups were significantly decreased(P <0.05~0.01),SOD was increased significantly (P <0.05),expression of VEGF and FN decreased significantly,and pathology score decreased significantly (P < 0.05). CONCLUSION Fushen Decotion can significantly improve the db/db mice renal damage and delay the development of diabetic nephropathy.%目的：观察复肾汤对 db／db 小鼠肾脏病变的影响。方法正常对照组为雄性 C57小鼠8只，常规饲养。雄性 db／db 鼠24只，正常饲养1周，血糖稳定升高4周后分为模型组、复肾汤小剂量组（L1）、复肾汤大剂量组（L2）。模型组灌胃给予生理盐水；L1组按 FST 13 mg／（kg·d）灌胃；L2组按 FST 39 mg／（kg·d）灌胃；连续8周，定期测体质量、血糖。实验结束后，采集血清，检测 T-chol、BUN、SOD、MDA 等生化指标；轻取肾脏，免疫组织化学法检测肾组织 VEGF、FN 的表达，光镜下观察肾脏组织病理变化。结果与模型组比较，L1、L2组的 T-chol、BUN、MDA 明显降低（P ＜0．05～0．01），SOD 明显升高（P ＜0．05）， VEGF、FN 表达明显下调，病理学评分明显降低（P ＜0．05）。结论复肾汤可以明显改善 db／db 小鼠肾脏损害，具有延缓糖尿病肾病病情发展的作用。