皮炎洗剂对小鼠慢性变应性接触性皮炎的影响及其作用机制

摘要

ABSTRACT:OBJECTIVE To study the efficacy of dermatitis lotion in the treatment of allergic contact dermatitis in mice. METHODS The 2,4-dinitro chlorobenzene(DNCB)was applied on the dorsum of mice to induce the allergic contact dermatitis models.60 mice were randomly divided into control,model(ACD),momestasone furoate,dermatitis lotion(0.4,0.2,0.1 g∕mL) groups,with 10 mice in each group.Mice were sensitized by DNCB and then treated topically with different drugs on le-sions.The inflammation degree of skin lesion were observed,histopathological analysis was measured by HE strain and the levels of serum IFN-γand IL-4 were detected by ELISA.RESULTS The dermatitis lotion of 0.4 g∕mL restrained the increase of thymus index,alleviated the inflammation degree of lesion on dorsum of mice and reduced the pathological score significant-ly,compared with the model group.In addition,the dermatitis lotion of 0.4 g∕mL adjusted the expression of serum IFN-γand IL-4 to return to normal levels.CONCLUSION These results suggest that dermatitis lotion can be used for the treatment of allergic contact dermatitis (ACD) mainly through the regulation Th1∕Th2 cytokines.%目的：评价皮炎洗剂治疗小鼠慢性变应性接触性皮炎(ACD)的疗效。方法采用2,4-二硝基氯苯(DNCB)诱导小鼠背部 ACD。60只小鼠随机分为空白对照组、ACD 组、阳性组、皮炎洗剂大剂量组(0.4 g/mL)、中剂量组(0.2 g/mL)、小剂量组(0.1 g/mL),每组10只,于致敏后给药,观察各组小鼠第4次激发24 h 后皮损炎症程度、组织病理切片、炎症细胞数量改变及血清中 IFN-γ和 IL-4水平的变化。结果皮炎洗剂中、大剂量能有效减轻 ACD 小鼠引起的免疫器官肿大、皮损炎症程度,减少皮损组织炎症细胞数量,调节 ACD 组小鼠血清中γ-干扰素(IFN-γ)和白细胞介素-4(IL-4)水平。结论皮炎洗剂对小鼠ACD 具有明显的治疗作用,其作用机制可能与皮炎洗剂调节 Th1、Th2型细胞因子有关。