活血通腑方对术后腹腔粘连大鼠Nrf2、HO-1及NQO1表达的影响

摘要

OBJECTIVE To investigate the effect of Huoxue Tongfu Formula on the expression of Nrf2,HO-1,NQO1 and mRNA in rats with postoperative peritoneal adhesion.METHODS Peritoneal adhesion was evaluated by gross abdominal ad-hesion score.The expressions of Nrf2,HO-1 and NQO1 were detected by western blot method.The expressions of Nrf2、HO-1 and NQO1 mRNA were tested via qPCR.RESULTS Huoxue Tongfu Formula could significantly reduce adhesion scores (P<0.01),manifested by evidently increased expression of Nrf2,HO-1 and NQO1 protein and mRNA(P<0.01).CON-CLUSION The expression of Nrf2,HO-1 and NQO1 in the adhesions of rats with peritoneal adhesion is significantly affected by Huoxue Tongfu Formula,which is able to resist peritoneal adhesion by regulating Nrf2 and its downstream factors and en-hancing the ability of anti-oxidative stress.%目的 探讨活血通腑方对术后腹腔粘连大鼠粘连部位Nrf2、HO-1及NQO1蛋白及mRNA的影响.方法 通过腹腔粘连评分评价腹腔粘连水平,Western blot法检测Nrf2核蛋白、HO-1和NQO1蛋白表达,qPCR检测Nrf2、HO-1和NQO1 mRNA表达.结果 活血通腑方能够显著降低肉眼腹腔粘连评分(P<0.01).活血通腑方组Nrf2核蛋白、HO-1和NQO1蛋白表达以及mRNA表达显著升高(P<0.01).结论 活血通腑方对术后腹腔粘连大鼠粘连部位Nrf2、HO-1及NQO1表达有显著影响,可能通过调节Nrf2及下游因子的表达进而提升抗氧化应激能力,发挥抗腹腔粘连效应.