消风宣窍汤对小鼠变应性鼻炎模型效应机制研究

摘要

目的 探讨消风宣窍汤对变应性鼻炎(AR)小鼠治疗效应及其相关机制.方法 48只BALB/c小鼠随机分为正常组,模型组,消风宣窍汤组,孟鲁斯特组,每组12只.采用OVA致敏建立小鼠AR模型.灌胃治疗1周,观测小鼠鼻部症状积分,体质量、胸腺和脾脏质量及指数,鼻黏膜组织学改变,血清总IgE、OVA特异性IgE以及胸腺IL-4、IL-17、IL-33、IFN-γ水平.结果 消风宣窍汤能够显著降低AR小鼠鼻部症状,减轻变应性反应引起的体质量、胸腺质量、胸腺指数的增加,能够显著减少血清总IgE、OVA特异性IgE的水平,抑制胸腺IL-4、IL-17、IL-33、IFN-γ产生,且在降低胸腺IL-33水平方面,消风宣窍汤效果优于西药孟鲁斯特组.结论 消风宣窍汤对AR小鼠疗效确切,其可能通过抑制胸腺细胞因子水平发挥抗AR效应.%OBJECTIVE To investigate the therapeutic effects of Xiaofeng Xuanqiao Decoction in treating mice with allergic rhinitis(AR)as well as its mechanism.METHODS 48 BALB/c mice were randomly divided into normal group,model group, Xiaofeng Xuanqiao Decoction group and montelukast group,with 12 mice in each group.OVA sensitized mice were established as the model with gavage for 1 week.Nasal symptom scores,weight,weight of thymus and spleen index,nasal mucosa chan-ges,serum total IgE,ova specific IgE and IL-4,IL-17,IL-33,IFN-γlevels in the thymus of mice were observed.RESULTS Xiaofeng Xuanqiao Decoction could significantly reduce nasal symptoms of AR in mice,reduce the increased weight,thymus weight and thymus index caused by allergic reaction.Besides,it could significantly reduce serum total IgE and ova specific IgE level,and inhibit the production of thymus IL-4,IL-17,IL-33,IFN-γ.In reducing the thymus IL-33 level,Xiaofeng Xuanqiao decoction was better than western medicine montelukast group.CONCLUSION Xiaofeng Xuanqiao Decoction has definite effects for AR in mice.The anti AR effects can be achieved by inhibiting the level of thymic cytokine.