NF-κB 通路与川芎提取物抗脑缺血作用相关性研究

摘要

目的：研究川芎提取物抗脑缺血的作用及 NF-κB 机制。方法大脑中动脉栓塞法制备大鼠脑缺血再灌注模型,实验组给予川芎提取物(100 mg/kg),每天灌胃给药1次,连续4周,模型组给予等量生理盐水,测定脑梗死面积,HE 染色法检测脑组织的损伤程度。流式细胞仪检测氧糖剥夺胶质细胞的凋亡率,Pathway Finder Array 法筛选出 NF-κB 信号通路被激活,免疫荧光法进一步检测 NF-κB p65亚基是否入核。结果与模型组相比,川芎提取物能减少脑缺血再灌注大鼠的脑梗死面积,降低 OGD 胶质细胞的凋亡率,NF-κB 信号通路在脑缺血再灌注后被激活,川芎提取物使 pP65进入细胞核内的数量显著降低。结论川芎提取物对大鼠脑缺血损伤有保护作用,可能与抑制 NF-κB 信号通路的激活有关。%OBJECTIVE To study the protective effects of Ligusticum Wallichii extract on cerebral ischemia and the mecha-nism.METHODS Male SD rats were divided into control group,model group and Ligusticum Wallichii extract(100 mg/kg) group.The rats in the treatment groups were administered once daily for 4 weeks.Middle cerebral artery occlusion method was used for cerebral ischemia-reperfusion model.After the model establishment,the neurological function scores were observed, the infarct size was measured by TTC staining,and the apoptosis rate of OGD glial cells by flow cytometry instrument,the ac-tivated NF-κB signaling pathway was measured Pathway Finder Array screening,the translocation of NF-κB p65 was measured by immunofluorescence.RESULTS Compared with the model group,Ligusticum Wallichii could significantly decrease the cer-ebral infarct size and the apoptosis rate of OGD glial cells Pathway Finder Array detection of NF-κB signaling pathways are ac-tivated in the normal saline group.Immunofluorescence test again important regulatory factors pP65 NF-κB signal is activated in the saline group into the nucleus.CONCLUSION The protection of extract from Ligusticum Wallichii on cerebral ischemia-reperfusion injury was related to NF-κB pathway.