目的:检测17β-estradiol对皮层海马神经元存活的影响,并判断其与nNOS/PSD95耦联的关系.方法:采用原代培养的ICR小鼠皮层海马神经元.培养7天后分溶剂对照组和给药组分别给予溶剂DMSO和浓度为10 μmol/L的17β-estradiol,LDH法判断细胞损伤,测定给药30 min后细胞死亡情况,并拍摄光镜照片观察细胞形态.同时利用免疫共沉淀法检测DMSO组,10nmol/L17β-estradiol组.10 μmol/L 17β-estradiol组的nNOS和PSD95的耦联情况,观察药物对其影响.结果:给药30 min后,与对照组相比,10 μmol/L 17 β-estradiol组LDH漏出率增加.同时nNOS/PSD95耦联增加,而10 nmol/L 17β-estradiol对nNOS/PSD95耦联并没有影响.结论:浓度为10 μmol/L的17β-estradiol会损伤皮层海马神经元,而且这一结果可能是通过促进nNOS/PSD95耦联实现的.%Objective:To detect the effects of 17β-estradiol on the survival of cortical hippocampal neurons and determine whether it is associated with nNOS/PSD95 complexes. Methods: ICR mice was adopted to culture cortical hippocampal neurons. Eight days later, lactate dehydrogenase(LDH) release rate were examined in cultured cortical hippocampal neurons exposed to DMSO or 10 μmol/L 17β-estradiol for 30 min. The neurons were photographed through a light microscope. Meanwhile, nNOS/PSD95 complexes of neurons treated with DMSO, 10 nmol/L 17β-estradiol and 10 μmo1/L 17β-estradiol were measured by co-immunoprecipitation. Results:At 30 minutes after administration, 10 μmol/L 17β-estradiol treatment increased LDH release rate and nNOS/PSD95 conjugating in cortical hippocampal neurons, and 10 nmol/L 17β-estradiol treatment had no influence on nNOS/PSD95 conjugating. Conclusion: 17β-estradiol of 10 μmol/L could injure cortical hippocampal neurons by promoting the conjugating of nNOS and PSD95.
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