胃癌中EphB3的表达及其对癌细胞增殖、侵袭能力的影响

摘要

Objective:To explore the expression of EphB3 in gastric cancer and its biological effects on cell prolif-eration and invasion. Methods:The expression of EphB3 in 60 specimens of gastric cancer tissues and 60 spceimens of adjacent tissues was detected by immunohistochemistry. The relationship between EphB3 expression and clinico-pathological features was statistically analyzed. The biological effects of overexpression of EphB3 on proliferation,inva-sion of gastric cancer cell were detected by CCK-8 and wound-healing assay. Results:According to IHC analysis, positive rates of EphB3 protein were significantly lower in gastric cancer than in adjacent tissue (P＝0. 004). EphB3 expression was associated with tumor differentiation,invasion depth and lymph node metastasis (P ＜0. 05 ). And EphB3 could inhibit proliferation and invasion in SGC7901 cell line. Conclusion:EphB3 can inhibit the tumorgene-sis,progression of gastric cancer and may become an important research direction of targeted therapy for gastric cancer in the future.%目的:研究EphB3在胃癌中的表达及其与临床病理特征的相关性,并探索EphB3对胃癌细胞增殖、侵袭能力的影响.方法:利用免疫组织化学染色法检测60例胃癌蜡块及配对的癌旁组织中EphB3的表达,并与临床病理特征进行相关性分析.转染过表达胃癌细胞株 SGC7901中 EphB3,通过 CCK -8实验检测EphB3对胃癌细胞增殖能力的影响.采用细胞划痕实验观察 EphB3对胃癌细胞侵袭能力的影响.结果:EphB3在胃癌中的阳性表达率明显低于癌旁组织(P＝0. 004),且与肿瘤分化程度、浸润深度、淋巴结转移存在相关性(P＜0. 05).转入外源性的EphB3显著降低细胞的增殖、侵袭能力.结论:EphB3能够抑制胃癌发生、发展的过程,可能成为未来胃癌靶向治疗的重要研究方向.