鼻咽NK/T细胞淋巴瘤肿瘤相关巨噬细胞与其增殖活性的关系

摘要

目的:研究鼻咽NK/T细胞淋巴瘤中肿瘤相关巨噬细胞(TAMs)数量与肿瘤增殖指数,以及2种巨噬细胞标志物(CD68与CD163)间的关系.方法:采用免疫组织化学染色法检测31例鼻咽NK/T细胞淋巴瘤和12例炎性反应病例的Ki67,CD68以及CD163.并对染色结果进行Pearson相关分析和t检验.结果:鼻咽NK/T细胞淋巴瘤中的TAMs数与肿瘤的增殖活性具有非常显著的正相关性(P=0.024),同时,CD163与CD68阳性细胞数密切相关(P =0.009),CD68的阳性率略高于CD163,但无统计学意义.鼻咽NK/T细胞淋巴瘤中TAMs的数量,与反应性病变相比具有明显差异(P＜0.05).结论:鼻咽NK/T细胞淋巴瘤中的TAMs与肿瘤细胞增殖活性密切相关,表明TAMs可促进NK/T细胞淋巴瘤细胞的增殖.并且2种标志物(CD68及CD163)均可识别TAMs.而CD163为TAMs的标志物似乎更加准确.%Objective; To explore the relationship between the number of tumor - associated macrophages (TAMs) and proliferative activity of tumor cells and the relationship between two macrophage biomarkers CD68 and CD163 in nasopharyngeal NK/T cell lymphoma. Methods: Immunohistochemistry was used to reconfirm the diagnosis of nasal NK/T - cell lymphoma and detect the numbers of TAMs and the ki - 67 label index of the tumor cells in all 31 patients. In addition, 12 cases of inflammatory cases were collected as controls, for which the immunostaining of CD68 and CD163 were done as well. Results;The number of TAMs was positively correlated with tumor proliferative activity( P =0.024) in nasopharyngeal NK/T cell lymphoma. The expression of CD68 and CD163 were closely related (P = 0.009), and the positive rate of CD68 was generally higher than CD163,however there was no statistical significance. Conclusion:The increase in numbers of TAMs in nasopharyngeal NK/T cell lymphoma often relates with higher proliferative index,indicating the TAMs play an important role in tumor proliferation. Meanwhile both CD68 and CD163 might be the markers for TAMs. But some evidence show the CD163 would be the better one.