γ-生育三烯酚对人胃癌 SGC-7901细胞的抑制作用及机制

摘要

目的 探讨γ -生育三烯酚(γ -tocotrienol)对人胃癌细胞SGC-7901抑制作用.方法 采用离体细胞培养技术,利用细胞生长曲线,单细胞凝胶电泳、琼脂糖凝胶电泳、透射电镜技术等方法,观察 γ-生育三烯酚对SGC-7901细胞生长抑制及其肿瘤细胞的损伤作用.结果 γ -生育三烯酚可明显抑制SGC-7901细胞增殖,抑制作用随作用浓度增加、作用时间延长而增强;并能引起人胃癌细胞株SGC-7901细胞DNA 分子的损伤以及细胞超微结构变化,如线粒体损伤和形成凋亡小体,且与γ-生育三烯酚作用的浓度存在一定的相关性.结论 γ -生育三烯酚对SGC-7901细胞生长有明显抑制作用,其抑制机制与参与DNA损伤、诱导细胞凋亡有关.%Objective:To investigate the effect of inhibitory growth on human gastric carcinoma SGC -7901 cells induced by 7 - tocotrienol. Methods: SGC -7901 cells were treated with different 7 - tocotrienol concentrations and with a negative control. The curve of cell growth, single cell gel electrophoresis, agarose gel electrophoresis and transmission electron microscopy were used in vitro. Results: y -Tocotrienol inhibited SGC -7901 cell growth in a concentration - and time - dependent manner. The inhibitory effects of SGC - 7 9 01 cells was correlated with theDNA - damage and the change of cell ultrastructure, such as mitochondrial injury and apoptotic bodies in a concentration - dependent manner at 48h. Conclusion: y - Tocotrienol could inhibit SGC - 7901 cells proliferation by the DNA - damagement and induced apoptosis. It may be one of the anticancer mechanisms of y - Tocotrienol.