人脐带血间充质干细胞对人胃癌MGC80-3细胞生物学行为的影响

摘要

目的：研究人脐带血间充质干细胞（ HUCB-MSC）对人胃癌MGC80-3细胞体外细胞外基质成分的影响，探讨其对胃癌细胞生物学行为的影响。方法：原代培养人脐带血间充质干细胞，流式细胞仪进行细胞表型鉴定。采用流式细胞仪半定量检测基质金属蛋白酶-7（ MMP-7）及基质金属蛋白酶抑制剂-1（ TIMP%Objective:To study the effects of human umbilical cord blood mesenchymal stem cells on extracellular matrixc of gastric carcinoma cells MGC80-3,to investigate its effects on cell biological behaviors of gastric carcino-ma. Methods:HUCB-MSCs were primary cultured in vitro and identified the phenotype. The protein level of MMP-7 and TIMP-1 were semi-quantitatively detected by flow cytometry. The mRNA level of MMP-7 and TIMP-1 were semi-quantitatively detected by reverse transcription PCR. Results:The expression of MMP-7 protein fluores-cence index( FI)was decreased with the increase of MSC-CM concentration. The expression of TIMP-1 protein fluorescence index was increased with the increase of MSC-CM concentration. The expression level of MMP -7, TIMP-1 in different experimental groups had significant statistical difference. The expression level of MMP-7 was decreased with the increase of MSC-CM concentration. The expression level of TIMP-1 was increased with the in-crease of MSC-CM concentration. There were significant differences between experimental groups and control group (P<0. 05). Conclusion:MSC-CM could inhibit the proliferation of human gastric cancer cell MGC80-3 through inhibiting the level of MMP-7 and upregulating the level of TIMP-1.