食管鳞癌组织中 CDK10基因启动子甲基化状态检测的临床意义

摘要

目的：探讨周期素依赖性激酶10（CDK10）在食管鳞癌中基因启动子甲基化状态及其与临床病理参数的关系。方法：应用甲基化特异性 PCR 检测 CDK10基因启动子在55例食管鳞癌组织及相应癌旁组织中的甲基化状态并结合临床病理资料进行分析。结果：在食管鳞癌组织中 CDK10甲基化阳性率（69．1％，38／55）显著高于癌旁对照组织（9．1％，5／55）（P ＜0．01）。CDK10甲基化与食管鳞癌淋巴结转移与临床分期显著相关（P ＜0．05）。结论：CDK10基因甲基化与食管癌淋巴结转移和临床分期密切相关。%Objective:To investigate the methylation status of the CDK10 gene promoter in esophageal squamous cell carcinoma.Methods:Methylation -specific PCR was performed to observe the CDK10 promoter methyation in 55 cases of esophagus carcinoma tissues and their adjacent non -cancerous tissues.The relationship between CDK10 methyation and clinical pathological parameters was also analyzed.Results:Methylated CDK10 was more frequently observed in primary tumors than in normal ovarian tissues (P <0.01).CDK10 methylation was also in relationship with lymph node metastasis and TNMstaging of tumors (P <0.05).Conclusion:CDK10 methylation may possess a significant function in the development of esophageal squamous cell carcinoma.