白藜芦醇对去势抵抗型前列腺癌22RV1细胞增殖与凋亡的影响

摘要

Objective:To observe the effect of resveratrol on proliferation and apoptosis of prostate cancer 22RV1 cells and explore the possible mechanisms.Methods:Ordinary optical inverted microscope was used to observe the morphological changes and cell proliferation was measured with a cell counting Kit -8.Apoptosis assays were per-formed by using the FITC Annexin V apoptosis detection Kit I by flow cytometry and Western blotting was used to an-alyze the expression of Bax and Bcl -2 protein in Resveratrol -treated 22RV1 cells.Accumulation of ROS was quan-tified by flow cytometry with a reactive oxygen species assay Kit.Results:Compared with the control group,the prolif-eration of 22RV1 was significantly inhibited by Res in a does and time -dependent manner(P <0.05),and the apop-tosis percentage in every experiment groups were higher(P <0.01 ),at the same time,the total ROS level was in-creased,and the expression of Bcl -2 was downregulated,as the Bax was upregulated with the increase of the Res concentration.Conclusion:Res could inhibit the proliferation cell and induced apoptosis of 22RV1,the mechanism is related with modulating ROS level and the expression of Bcl -2 /Bax.%目的：探讨白藜芦醇（resveratrol，Res）对去势抵抗型前列腺癌（castration －resistant prostate cancer， CRPC）22RV1细胞增殖与凋亡的影响并探讨其可能机制。方法：用不同浓度 Res 作用于22RV1细胞24h 后，在光学显微镜下观察细胞形态学变化。CCK －8法检测 Res 对细胞增殖的影响。应用 Annexin －V ／PI 双标记法染色后，用流式细胞仪检测细胞凋亡情况。用活性氧特异性试剂盒检测活性氧含量。Western blot 检测 Bcl－2和 Bax 蛋白表达情况。结果：与对照组比较，Res 可使22RV1细胞形态出现皱缩，变圆，细胞间隙增大，贴壁细胞减少，凋亡细胞增多。CCK －8结果示 Res 可抑制22RV1细胞增殖作用（P ＜0．05）。流式检测结果示Res 可诱导22RV1细胞凋亡（P ＜0．01）。活性氧检测结果示 Res 可增加22 RV 1细胞内活性氧含量（P＜0．01），并呈剂量相关性。Western blot 检测发现随 Res 浓度的增加，Bcl －2蛋白表达下降，Bax 蛋白表达增加。结论：Res 能够抑制22RV1细胞的增殖并诱导其凋亡。Res 诱导22RV1细胞凋亡的机制可能与 Res 增加22RV1细胞内活性氧水平，激活促凋亡因子 Bax，并抑制抗凋亡因子 Bcl －2的活性，继而引发后续凋亡反应。