Objective To study the expression levels of Ezrin and P53 in colorectal carcinoma and their clinicopathologic al significance. Methods The tissue microarray and immunohistochemical staining technology were used to detect the expression of Ezrin and P53 in 109 cases of colorectal carcinoma tissues. The correlation between Ezrin and P53 expressions and their relation with the clinicopathological characteristic of colorectal carcinoma were analyzed by using the statistical method. Results The Ezrin protein was highly expressed in the colorectal carcinoma tissues ,the positive expression rate in the TNM stageⅢ,Ⅳgroup was 89.55%(60/67),which was higher than 23.81%(10/42) in the TNM stageⅠ,Ⅱgroup,the expression positive rate in the low differentiation and non-differentiation group was 94.44%(17/18),which was higher than 58.24%(53/91)in the high and middle differentiation group,the positive rate in the T3,T4 group was 67.31%(70/104),which was higher than 0 (0/5) in the T1,T2 group,the positive rate in the lymph node metastasis group was 89.55%(60/67),which was higher than 23.81%(10/42) in the non-lymph node metastasis group,the differences were statistically significant(P<0.05);its expression had no relation with the sex and age. P53 was highly expressed in the colorectal carcinoma tissues ,the expression positive rate in the TNM stageⅢ, Ⅳgroup was 53.73%(36/67),which was higher than 23.81%(10/42) in the TNM stageⅠ,Ⅱgroup,the positive rate in the low differentiation and non-differentiation group was 72.22%(13/18),which was higher than 36.26%(33/91) in the high and middle differentiation group,the positive rate in the lymph node metastasis group was 53.73%(36/67),which was higher than 23.81%(10/42) in the non-lymph node metastasis group,the differences were statistically significant(P<0.05);its expression had no relation with the sex,age and infiltration degree. The Ezrin expression in the colorectal carcinoma had no relation with P53 (r=0.212,P>0.05). Conclusion Applying the tissue microarray for efficiently detecting clinical tissue samples in large scale is feasible and has the advantages of rapidness,convenience,economy and accuracy. The Ezrin protein may be a useful indicator for predicting the metastasis of colorectal carcinoma;the combined detection of Ezrin and P53 may serve as an effective indicator for judging the prognosis of colorectal carcinoma and screening the metastasis of colorectal carcinoma ,which is conducive to the judgment of colorectal carcinoma prognosis.%目的探讨Ezrin和P53在结直肠癌中的表达及意义。方法应用组织芯片和免疫组织化学技术检测109例结直肠癌组织中Ezrin和P53蛋白表达，并应用统计学方法分析Ezrin和P53表达水平与结直肠癌各种临床病理特征的关系及二者相关性。结果 Ezrin蛋白在结直肠癌组织中高表达， TNM分期Ⅲ、Ⅳ期组表达阳性率[89.55%（60/67）]高于Ⅰ、Ⅱ期组[23.81%（10/42）]，低分化、未分化组表达阳性率[94.44%（17/18）]高于高分化、中分化组[58.24%（53/91）]， T3、T4组阳性率[67.31%（70/104）]高于T1、T2组[0（0/5）]，有淋巴结转移组表达阳性率[89.55%（60/67）]高于无淋巴结转移组[23.81%（10/42）]，差异均有统计学意义（P<0.05）；其表达与性别、年龄无关。P53在结直肠癌中高表达，TNM分期Ⅲ、Ⅳ期组表达阳性率[53.73%（36/67）]高于Ⅰ、Ⅱ期组[23.81%（10/42）]，低分化、未分化组表达阳性率[72.22%（13/18）]高于高分化、中分化组[36.26%（33/91）]，有淋巴结转移组表达阳性率[53.73%（36/67）]高于无淋巴结转移组[23.81%（10/42）]，差异均有统计学意义（P<0.05）；其表达与性别、年龄、浸润深度无关。结直肠癌组织中Ezrin表达与P53无关（r=0.212，P＞0.05）。结论应用组织芯片大规模高效检测临床组织样本是可行的，具有快速、方便、经济、准确等特点。Ezrin蛋白可能是预测结直肠癌转移的有用指标；联合检测Ezrin、P53蛋白可作为判断结直肠癌预后和筛选高危结直肠癌转移患者的有效指标，有助于结直肠癌预后的判断。
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