重度畸形精子症患者精核蛋白表达的研究

摘要

Objective To investigate the difference of content and expression of protamine (TNBP) in the severe terato-zoospermia patients and provide the theoretical basis. Methods It was extracted TNBP of semen protamine from 23 severe tera-tozoospermia patients(the severe teratozoospermia group) and 37 healthy men(the control group) from Feruary 2013 to May 2014 with polyacrylamide gelelectrophoresis and then confirm the relative content of proein bands scanned by micro imaging density measuring instrument under the cataphoresis in the polyacrylamide hydrogel. Results The value of TH/TP,TNBP1/TNBP2,TP/TNBP2 in the evere teratozoospermia group were significantly higher than those of the control group (P<0.05). Compared with the control group,there was no significant difference in TNBP1/TP value(P>0.05). Thesperm density was lower than that of the control group(P<0.05). Both the sperm survival rate and the precession movement rate showed no significant difference with the control group(P>0.05). Conclusion The abnormity of TNBP1/TNBP2,TP/TNBP2 in the relative content of TNBP is one of the reasons leading to severe teratozoospermia.%目的研究重度畸形精子症患者精核蛋白（TNBP）含量及表达差异，为临床治疗提供理论基础。方法2013年2月至2014年5月提取23例重度畸形精子症患者（重度畸精组）和37例健康男性（对照组）的精液TNBP，在聚丙酰胺凝胶中电泳，经微显像测密仪扫描以测定各蛋白条带的相对含量。结果重度畸精组总组蛋白/总碱性蛋白（TH/TP）、1型精核蛋白/2型精核蛋白（TNBP1/TNBP2）、TP/TNBP2值显著高于对照组，差异均有统计学意义（P<0.05）；而TNBP1/TP值与对照组比较，差异无统计学意义（P>0.05）；精子密度显著低于对照组，差异有统计学意义（P<0.05）；精液活率、前向运动率与对照组比较，差异均无统计学意义（P>0.05）。结论 TNBP的相对含量中TNBP1/TNBP2和TP/TNBP2比值异常是导致重度畸精症的原因之一。