Objective To explore the protective effect of intravenous hydrogen saturated saline on sodium taurocholate induced rat severe acute pancreatitis associated lung injury (APALI). Methods (1)The establishment and treatment of severe APALI model:54 healthy male SD rats were randomly divided into the sham operation group (Sham group),model group[severe a-cute pancreatitis(SAP)+normal saline(NS) group ] and hydrogen water treatment group(SAP+H2 group),18 cases in each group;each group was subdivided into 3 subgroups according to the time points of 6 ,12,24 h,6 cases in each subgroup. In the Sham group,the pancreas was stirred for several times after laparotomy and then the abdominal cavity was closed without conducting oth-er treatment. In the SAP+NS group and SAP+H2 group,5%sodium taurocholate(1 mL/kg) was retrogradely injected by the biliary-pancreatic duct opening (0.1 mL/min) for constructing the model,5 mL/kg normal saline or the equivalent hydrogen saturated saline was respectively injected by the tail vein at 1 h after successfully constructing the model. (2)Rats in each group were killed for collecting serum at the time points of 6,12,24 h. The serum TNF-αand IL-1βlevels were detected by ELISA. Results (1) The TNF-αlevels at 6,12,24 h were (215.3±6.0),(263.2±7.4),(288.0±5.6)ρg/mL in the SAP+NS group and (204.1±8.5), (122.4±10.3),(84.1±8.6)ρg/mL in the SAP+H2 group,which were higher than (58.8±7.5),(57.5±5.1),(59.6±6.0)ρg/mL in the Sham group,moreover the TNF-αlevel at 3 time points in the SAP+NS were higher than those in the SAP+H2 group,the differences were statistically significant(P<0.05). (2)The IL-1βlevels at 6,12,24 h were(152.8±9.8),(215.4±10.4),(254.3±8.6)ρg/mL in the SAP+NS group and (154.7±6.8),(80.3±8.3),(59.3±8.2)ρg/mL in the SAP+H2 group,which were higher than(39.8±3.3),(39.5± 3.3),(40.5±5.2)ρg/mL in the Sham group,moreover which at 12,24 h in SAP+NS group were higher than those in the SAP+H2 group,the differences were statistically significant(P<0.05),while the IL-1β level at 6 h had no statistical difference between the SAP+H2 group and SAP+NS group(P>0.05). Conclusion The tail vein injection of hydrogen saturated saline has certain treatment and protective effect on APALI.%目的探讨经尾静脉注射氢饱和生理盐水对牛磺胆酸钠诱导的大鼠重症急性胰腺炎相关性肺损伤（A-PALI）的保护作用。方法（1）大鼠重症APALI模型的建立及处理：将54只健康SD雄性大鼠随机分为假手术组（Sham组）、模型组[重症急性胰腺炎（SAP）+氯化钠注射液（NS）组]和氢水处理组（SAP+H2组），每组18只；各组再按6、12、24 h 3个时间点分为三组，每组6只。Sham组大鼠开腹翻动胰腺数次后随即关腹，不作其他处理，SAP+NS、SAP+H2组以5%牛磺胆酸钠（1 mL/kg）经胆胰管开口逆行注入（0.1 mL/min）建立模型，在建模成功后1 h经尾静脉分别注射5 mL/kg生理盐水或等量氢饱和生理盐水。（2）各组分别在6、12、24 h 3个时间点处死大鼠，收集血清，用酶联免疫吸附试验（ELISA法）检测血清TNF-α、IL-1β水平。结果（1）SAP+NS、SAP+H2组血清中TNF-α水平在6、12、24 h各个时间点[SAP+NS组：（215.3±6.0）、（263.2±7.4）、（288.0±5.6）ρg/mL，SAP+H2组：（204.1±8.5）、（122.4±10.3）、（84.1±8.6）ρg/mL]均高于Sham 组[（58.8±7.5）、（57.5±5.1）、（59.6±6.0）ρg/mL]，且SAP+NS 组在3个时间点的TNF-α水平均高于SAP+H2组，差异均有统计学意义（P<0.05）。（2）SAP+NS、SAP+H2组血清中IL-1β水平在6、12、24 h各个时间点[SAP+NS 组：（152.8±9.8）、（215.4±10.4）、（254.3±8.6）ρg/mL，SAP+H2组：（154.7±6.8）、（80.3±8.3）、（59.3±8.2）ρg/mL]均高于Sham 组[（39.8±3.3）、（39.5±3.3）、（40.5±5.2）ρg/mL]，且SAP+NS组在12、24 h时IL-1β水平均高于SAP+H2组，差异均有统计学意义（P<0.05），而SAP+H2组6 h的IL-1β水平与SAP+NS组比较，差异无统计学意义（P＞0.05）。结论经尾静脉注射氢饱和生理盐水对APALI有一定治疗保护作用。
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