ELISA法定性试验室内质控方法探讨

摘要

Objective To discuss ELISA qualitative test inside quality control method. Methods Detected HBsAg,HBsAb,HBeAg,HBeAb and HBcAb of weakly positive oc matter and 900 patients serum by ELISA method,analyse QC data with Levey-Jennings and half hold range method. Results 25 patients serum in 900 patients serum appeared false-negative and false-positive alone utilize Levey-Jennings QC map. Conclusion Combine Levey-Jennings oc map and half hold range method wben utilize ELISA qualitative test interior quality control method,reasonable choose fit experimental QC method by experimentation purpose.%目的 探讨ELISA法定性试验室内质控方法.方法 以ELISA法检测弱阳性质控品和900份病人血清的HBsAg,HBsAb,HBeAg,HBeAb和HBcAb,用Levey-Jennings质控图法和半固定范围法分别分析质控数据.结果 单独应用Levey-Jennings质控图法时,900份血清中有25份标本阴阳性出现误判.结论 在应用ELISA法定性试验室内质控方法时,结合Levey-Jennings质控图法和半固定范围法,根据实验目的合理选择适合各实验的质控方法.