南京地区变应性鼻炎患者吸入性变应原特异性IgE和总IgE的检测分析

摘要

目的 检测和分析不同年龄变应性鼻炎(AR)患者血清总IgE水平和吸入性变应原分布特点.方法 采用速率散射比浊法和免疫印迹法分别检测351例(三组)不同年龄(4～16岁,17～40岁和≥41岁)患者血清中总IgE水平和吸入性变应原特异性IgE抗体.结果 4～16岁AR患者血清总IgE增高率(77.31％)及水平(488.31±341.41 IU/ml)显著高于其他两组患者结果(63.1％,368.42±336.62 IU/ml,P＜0.01和40.0％,287.54±315.06 IU/ml,P＜0.001).351例AR患者特异性IgE抗体-吸入性变应原分布率依次为:屋尘螨(81.48％)、粉尘螨(81.2％)、动物毛发(猫/狗)(38.46％)、蟑螂(25.36％)、花粉组合(13.11％),艾蒿(11.97％)、矮豚草(11.11％)、悬铃树(7.12％)、霉菌组合(6.27％)和桦树(1.14％).结论 少儿患者组血清中总IgE水平和对粉尘螨、屋尘螨、动物毛发过敏的比例显著升高.40岁以上患者组免疫功能减低可能影响总IgE产生.诱发该地区不同年龄组AR的主要过敏原(阳性率超过20％以上)是粉尘螨、屋尘螨、动物毛发(猫/狗)和蟑螂.%Objective To detect the IgE level and aeroallergen specific IgE in order to analyze the characteristic in allergic rhinitis patients of different age. Methods Serum aeroallergen specific IgE antibody and IgE level of patients were detected by velocity scatter nephelometry and immunoblotting. 351 serum samples velocity scatter nephelometry and immunoblotting. 351 serum samples of patients were stratified three age groups :4~ 16 years> 17～40 years old and greater than 40 years old. Results The total IgE positive rate (77. 31%) and IgE level(488. 31 ± 341. 41 IU/ml) in 4~16 years group were significantly higher than that two groups (368. 42 ± 336. 62 IU/ml and 287. 54 ± 315. 06 IU/ml, P<0. 01 and 0. 001). The positive rate of aeroallergen specific IgE antibody in 351 patients serum with allergic rhinitis in order were dermatophagoides pteron-yssinus (81. 48%) , dermatophagoides farinae (81. 2%) , cat-dog epithelia (38. 46%) , cockroach(25. 36%) , pollen mixture (13. Ll%),mugwort(ll. 97%) ,ragweed(ll. 11%),plane tree(7. 12%),mould mixture (6. 27%) and birch(1. 14%). Conclusion The total IgE level in junior group was significant elevated and the sensitive to aeroallergen (dermatophagoides pteronyssinus, dermatophagoides farinae and cat-dog epithelia) was also reinforced. The immunoreactionin of hyper-forty paient group was decreased and It would affect total IgE production. The main aeroallergens (over 20%) of induction different age patients with allergic rhinitis in Nanjing area were mites,cat-dog epithelia and cockroach.