HCV核心抗原检测在血液筛查中的初步研究

摘要

Objective To evaluate the feasibility of hepatitis C-cAg enzyme-linked immunosorbent (EIA) method in blood screening. Methods The samples collected from donors were tested by anti-HCV EIA and HCV-cAg EIA, and the samples for anti-HCV negative were further detected by Chiron Procleixa TMA again. Results In 1 762 donor samples,the positive rate of anti-HCV and HCV-cAg were 12(0. 68 %), 10(0. 57%) respectively, and 4(0. 23 %) of the samples were positive bothly. In 1 750 anti-HCV negative samples,the HCV-cAg positive samples were 6,but none was positive detected by Chiron Procleixa TMA. Conclusion The hepatitis C-cAg enzyme-linked immunosorbent kits may be useful in blood screening in order to reduce the risk of HCV-transmitted diseases.%目的 探讨丙型肝炎病毒核心抗原(HCV cAg)醇免检测试剂用于血液筛查的应用价值.方法 同时用抗HCV抗体和HCV cAg酶免检测试剂平行筛查血液,对HCV抗体阴性样本用Chiron Procleixa TMA系统检测HCV RNA.结果 1 762份无偿献血者样本中,抗HCV阳性12份(0.68％),HCV cAg阳性10份(0.57％),阳性检出率差异无统计学显著性意义(P＞0.05),抗HCV及HCV cAg同时阳性4份(0.23％).1 750份抗HCV阴性样本中,其中HCV cAg阳性样本6份,HCV RNA全为阴性.结论 在血液筛查中增加HCV cAg检测,对降低HCV的输血传播危险具有一定价值.